Beat the limits of Phototoxicity of light

Phototoxicity describes the phenomenon that light induces undesirable physiological changes in the observed living sample, be that single cells from a cell culture or a multicellular organism. With the excitation light intensities widely used in live cell imaging, it is critical to create new optical tools that will remove significant alterations in the biochemistry, physiology and dynamic behaviour of the observed sample from light.

Our team has developed a host of live cell imaging tools that beats the phototoxicity limits of light using computational and advanced laser scanning approaches.

"Tunable Non-interferometric Widefield Contrast" (In Prep) (Published Abstract)
"Combined scattering, interferometry and fluorescence oblique illumination for live cell nanoscale imaging " ACS Photonics (2022)
"Modified inverted selective plane illumination microscopy for sub-micrometer imaging resolution in polydimethylsiloxane soft lithography devices" Lab On Chip 20, 3960-3969(2020)
"Holo-UNet: hologram to hologram neural network restoration for high fidelity low light quantitative phase imaging of live cells" Biomedical Optics Express 11, 5478 (2020)
"Raster Scanning Adaptive Optics for video rate correction and large field of view imaging” Biomedical Optics Express 2 1032 (2020)
"In situ retrieval and correction of aberrations in moldless lenses using Fourier Ptychography ", Optics Express 26 2708 -2719 (2018)
Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging” Journal of Biophotonics (2017)
"Automated Fourier space recognition filtering for off-axis digital holographic microscopy", Biomed. Opt. Express 7(8), 3111-3123 (2016)